Not all important health related questions could be answered using the data collected in NBS1. Therefore, in October/November 2005 we have approached all the NBS1 participants who had given permission for further research and asked them to fill out an additional questionnaire (NBS2 QN). This QN contained questions about a number of topics, including health and disease, pregnancy, mood and behaviour, daily activities, and memory. In addition, a part of these participants was asked to undergo supplementary examinations in the RUNMC, dependent on age, sex and questionnaire information. This supplementary examination consisted of, a.o., a food frequency questionnaire (FFQ), blood pressure measurements, measurement of length and weight, donation of a number of (fasting) blood samples and a urine sample, non-invasive measurements of atherosclerosis, cognitive tests, and a gait analysis.


Several departments of the RUNMC were involved in the second phase of the NBS: General Internal Medicine, Endocrinology, Geriatrics, Neurology, Nephrology, Psychiatry, Rheumatology and Vascular Surgery. In addition, collaboration with the department of Human Nutrition of Wageningen University and Research Centre took place. All these departments have financially invested in NBS2.


Below you can find an overview of the NBS2 per age category (based on age at November 1 2005) and the number of participants. For detailed information on the NBS2 see sections ‘Researcher data'.


Age below 50

2926 NBS2 QNS were sent out, of which 1884 (64%) were completed. Between the years 2008 and 2010 people below the age of 50 were invited to fill out a food frequency questionnaire and to donate a blood and urine sample. This part of the study has been named NBS3.


Age 50 to 70

In this age group 2807 QNS were sent out; the response was 75% (2114 QNS). In addition, 1491 people participated in an extensive study from the department of Internal Medicine of the RUNMC: the NIMA study. For this study, participants visited the RUNMC once for donation of a blood sample, measurement of length, weight, blood pressure, and waist and hip circumference. During this same visit four different non-invasive measurements of atherosclerosis were performed, i.e. intima media thickness (IMT), endothelium function using flow-mediated dilatation (FMD), ankle-brachial index (ABI) and pulse-wave velocity (PWV). A large part of these participants has also filled out a FFQ (1168).


In the meantime, a pilot for a follow-up study in this population has been performed: NBS-NIMA2. The pilot study, executed in 2011, included repeated measurements of NIMA (including FMD, IMT, the presence and thickness of plaques in the carotid arteries, ABI at rest and after exercise, PWV, and PWA), measurements of traditional and new clinical and biochemical measurements, including a questionnaire on life style habits and medical history, and evaluation of clinical endpoints. Venous blood was drawn and a urine sample was collected. The measurement protocol was exactly the same as in the NBS-NIMA 1 study. Hundred NBS2-NIMA1 participants were invited for the pilot; 95 individuals participated.


Age over 70

Of the 2253 NBS2 QNS that were sent out in this age category, 1596 have been filled in: a response of 71%. Also, 150 FFQs were sent out, of which 77 were filled in (response 51%). A selection of 300 people was approached for additional examinations by the department of Geriatrics, including cognitive tests, quantitative gait analysis, functional mobility tests and measurement of hand grip strength; one hundred people (33%) have participated.